AffiPCR® Universal SYBR qPCR Master Mix Passive ROX

AffiPCR® Universal SYBR qPCR Master Mix Passive ROX

AffiPCR® Universal SYBR qPCR Master Mix Passive ROX is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The core component AceTaq DNA Polymerase is a chemically modified hot-start DNA polymerase. Combined with the optimal Buffer optimized for qPCR, nonspecific amplification can be effectively inhibited, thus significantly improving the amplification efficiency, which is suitable for high-sensitivity qPCR detection. This product is a dye-based 2X master mix. A good standard curve can be obtained in a wide quantitative area, and the target gene can be accurately quantified and detected, with good repeatability and high reliability. This product contains a unique ROX Passive Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.

Application:

It is applicable for DNA quantification from various type of templates such as genomic DNA, cDNA, plasmid DNA and λ DNA.

Storage Conditions:

Store at -30 ~ -15°C and protect from light. Transport at ≤0°C.

Applications:

It is applicable for DNA quantification from various type of templates such as genomic DNA, cDNA, plasmid DNA and λ DNA.

Applicable Instruments:

Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo 4; Cepheid SmartCycler; Eppendorf Mastercycler ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science LightCycler 480; Thermo Scientific PikoReal Cycler; Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus; Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P.